Molecular Biology: DNA Sequencing, Oligonucleotide Synthesis and qPCR


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DNA Sequencing | Customized Services | Quantitative PCR

CFG DNA Sequencing Services

Template Concentrations | Primer Concentrations | Policy on Repeat Reactions
Quality Control | Standard Primers | Template Preparation

Template Concentrations

Template	Concentration	Template	Concentration
Plasmid	50 - 100 ng/µL	PCR fragment
Cosmid	0.5 - 1.0 µg/µL	100 - 200 bp	3 ng/µL
Bac	0.5 - 1.0 µg/µL	200 - 500 bp	10 ng/µL
Bacterial Genomic	2.0 - 3.0 µg/µL	500 - 1,000 bp	20 ng/µL
Phage	0.5 - 1.0 µg/µL	1,000 - 2,000 bp	40 ng/µL
		> 2,000 bp	100 ng/µL

Please include enough template for reactions to be repeated at least one time with four times the amount of template. For plasmid templates, we can accept concentrations within the range of 100 - 300 ng/µL. The more concentrated the sample, the more flexibility we have in adjusting the concentration of repeat reactions, if necessary. All templates submitted by an individual in an order must be at the same concentration. Thus, if you are submitting 5 plasmids, and their concentrations range from 500 ng/µL down to 100 ng/µL, the samples should be diluted such that they can all be submitted at a concentration of 100 ng/µL. Submission of a batch of samples that are not at a uniform concentration may result in a delay in the processing of your samples.

DNA Sequencing | Customized Services | Quantitative PCR